Determining the Q10 of Endothermic and Ectothermic Organisms: Teacher Information
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Respirometers
To make the respirometers, the following is needed:
- # 7 stopper with two holes
- a third hole is needed to add a thermometer
- 32 oz Gatorade bottles
- Surgical, flexible tubing
- L-shaped glass tubing-this can be made by using a Bunsen burner
- U-shaped glass tubing-this can be made by using a Bunsen burner
- Thermometer
- 20 cc syringe
- Soda lime
- Cheese cloth
- Rubber band
- Colored water
The respirometer can be made as diagramed above.
Soda lime packets are made by adding a teaspoon of soda lime in several squares of cheese (3" x 3") cloth or tied off with a rubber band. The soda lime can be used several times but once the packets are made should be kept in a air tight bag until used so that the carbon dioxide in the air does not combine with the soda lime. Soda lime can be purchased at a chemical supply company.
The stoppers, glass tubing, and surgical tubing can be bought at a science supply company.
The stopper needs to have a third hole drilled in it to accommodate a thermometer.
The syringes can be obtained at a medical supply company
The layers of screen is needed to keep the animal away from the soda lime as it is corrosive. The screen can be purchased at a hardware store.
